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. 2006 Nov 17;189(3):1145–1149. doi: 10.1128/JB.01061-06

FIG. 1.

FIG. 1.

Western blot using specific antibodies against RamB and cell extracts of C. glutamicum WT (100 μg total protein) grown in minimal medium containing glucose (Glc), glucose and acetate (Glc/Ac), or acetate (Ac). The presence of RamB in the extracts was tested by incubation with specific mouse antibodies followed by incubation with peroxidase-conjugated goat anti-mouse immunoglobulin G Fab fragments and visualization by chemiluminescence. Purified RamB protein (RamBx6His; 0.2 μg) was used as a control.