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. 2006 Nov 17;189(3):1145–1149. doi: 10.1128/JB.01061-06

FIG. 2.

FIG. 2.

Genomic locus of the ramB promoter region and DNA fragments used for mapping the relevant RamA and RamB binding loci (A) and representative EMSAs using various DNA fragments and RamA protein (B) and RamB protein (C). sdhC codes for a protein annotated as a membrane anchor protein of the succinate dehydrogenase. The transcriptional start sites for the ramB gene and the sdhC gene are designated TS ramB and TS sdhC, respectively. (A) The RamB binding site is designated as a 13-bp motif. The nucleotide sequence of the putative RamA binding site is given above the black box. The putative DtxR binding site (DtxR bs) is indicated by a dark gray box. The numbers indicate the positions of the centers of these sites relative to the ramB TS site. The fragments used for the binding assays are shown as narrow bars. Also indicated are binding (+) and lack of binding (−) of RamA and RamB. Lanes 1 to 4 (B and C) show EMSAs using 0, 0.25, 0.5, and 1.0 μg of RamA or RamB, respectively.