TABLE 1.

Strains, plasmids and oligonucleotide primers

Name	Description	Reference or source
N. meningitidis strains
MC58	Wild-type serogroup B strain	14
nsrR	Derivative of MC58 with insertion of spectinomycin resistance cassettte into nsrR	This work
aniA	Derivative of MC58 with insertion of spectinomycin resistance cassette into aniA	23
norB	Derivative of MC58 with insertion of spectinomycin resistance cassette into norB	1
fnr	Derivative of MC58 with insertion of erythromycin resistance cassette into fnr	23
MC58 PaniA-lacZ	Derivative of MC58 containing an aniA promoter-lacZ fusion	23
aniA PaniA-lacZ	Derivative of N. meningitidis aniA containing an aniA promoter-lacZ fusion	1
nsrR PaniA-lacZ	Derivative of N. meningitidis nsrR containing an aniA promoter-lacZ fusion	This work
fnr PaniA-lacZ	Derivative of N. meningitidis fnr containing an aniA promoter-lacZ fusion	23
nsrR fnr PaniA-lacZ	Derivative of N. meningitidis nsrR transformed with fnr genomic DNA and containing an aniA promoter-lacZ fusion	This work
MC58 PnorB-lacZ	Derivative of MC58 containing a norB promoter-lacZ fusion	This work
nsrR PnorB-lacZ	Derivative of N. meningitidis nsrR mutant strain containing an norB promoter-lacZ fusion	This work
fnr PnorB-lacZ	Derivative of N. meningitidis fnr containing an norB promoter-lacZ fusion	This work
nsrR fnr PnorB-lacZ	Derivative of N. meningitidis nsrR transformed with fnr genomic DNA and containing an norB promoter-lacZ fusion	This work
Plasmids
pHP45Ω	Contains Ω cassette encoding spectinomycin resistance	21
pLES94	Promoter-probe vector for neisserial promoters	27
pJR113	pGemT-easy vector containing nsrR with deletion and allelic disruption using Ω cassette	This work
pLES-PaniA-lacZ	aniA-lacZ translational fusion vector	23
pLES-PnorB-lacZ	norB-lacZ translational fusion vector	This work
pCR-BluntII-TOPO	Vector for cloning blunt-ended PCR products	Invitrogen
Oligonucleotide primers
NOregF1	5′-CAGGGTTGTGGCAAAAGCGGT-3′; for amplification of nsrR gene and flanking region	This work
NOregR1	5′-ACCACCTGACCCTGATGTGC-3′; for amplification of nsrR gene and flanking region	This work
NoregInv1	5′-AAAAGCTTCGTATCTGGACGGTTTCACGCTCC-3′; for inverse PCR deletion and allelic disruption of nsrR gene	This work
NoregInv2	5′-AAAAGCTTCCAGCGCATCGTCGTTGATGGC-3′; for inverse PCR deletion and allelic disruption of nsrR gene	This work
MFA7	5′-GGGATCCCGTTTCATAATGTTTTCCTTTTGT-3′; for amplification of norB-aniA promoter region	23
MFA8	5′-GGGATCCCGTCCCATTTTGAGAGCTCCTTTT-3′; for amplification of norB-aniA promoter region	23
MFA10	5′-GTGCTGCAAGGCGATTAAGTT-3′; for checking orientation of insertion of norB-aniA promoter in lacZ fusions	This work