N. meningitidis strains |
|
|
MC58 |
Wild-type serogroup B strain |
14 |
nsrR
|
Derivative of MC58 with insertion of spectinomycin resistance cassettte into nsrR
|
This work |
aniA
|
Derivative of MC58 with insertion of spectinomycin resistance cassette into aniA
|
23 |
norB
|
Derivative of MC58 with insertion of spectinomycin resistance cassette into norB
|
1 |
fnr
|
Derivative of MC58 with insertion of erythromycin resistance cassette into fnr
|
23 |
MC58 PaniA-lacZ
|
Derivative of MC58 containing an aniA promoter-lacZ fusion |
23 |
aniA PaniA-lacZ
|
Derivative of N. meningitidis aniA containing an aniA promoter-lacZ fusion |
1 |
nsrR PaniA-lacZ
|
Derivative of N. meningitidis nsrR containing an aniA promoter-lacZ fusion |
This work |
fnr PaniA-lacZ
|
Derivative of N. meningitidis fnr containing an aniA promoter-lacZ fusion |
23 |
nsrR fnr PaniA-lacZ
|
Derivative of N. meningitidis nsrR transformed with fnr genomic DNA and containing an aniA promoter-lacZ fusion |
This work |
MC58 PnorB-lacZ
|
Derivative of MC58 containing a norB promoter-lacZ fusion |
This work |
nsrR PnorB-lacZ
|
Derivative of N. meningitidis nsrR mutant strain containing an norB promoter-lacZ fusion |
This work |
fnr PnorB-lacZ
|
Derivative of N. meningitidis fnr containing an norB promoter-lacZ fusion |
This work |
nsrR fnr PnorB-lacZ
|
Derivative of N. meningitidis nsrR transformed with fnr genomic DNA and containing an norB promoter-lacZ fusion |
This work |
Plasmids |
|
|
pHP45Ω |
Contains Ω cassette encoding spectinomycin resistance |
21 |
pLES94 |
Promoter-probe vector for neisserial promoters |
27 |
pJR113 |
pGemT-easy vector containing nsrR with deletion and allelic disruption using Ω cassette |
This work |
pLES-PaniA-lacZ
|
aniA-lacZ translational fusion vector |
23 |
pLES-PnorB-lacZ
|
norB-lacZ translational fusion vector |
This work |
pCR-BluntII-TOPO |
Vector for cloning blunt-ended PCR products |
Invitrogen |
Oligonucleotide primers |
|
|
NOregF1 |
5′-CAGGGTTGTGGCAAAAGCGGT-3′; for amplification of nsrR gene and flanking region |
This work |
NOregR1 |
5′-ACCACCTGACCCTGATGTGC-3′; for amplification of nsrR gene and flanking region |
This work |
NoregInv1 |
5′-AAAAGCTTCGTATCTGGACGGTTTCACGCTCC-3′; for inverse PCR deletion and allelic disruption of nsrR gene |
This work |
NoregInv2 |
5′-AAAAGCTTCCAGCGCATCGTCGTTGATGGC-3′; for inverse PCR deletion and allelic disruption of nsrR gene |
This work |
MFA7 |
5′-GGGATCCCGTTTCATAATGTTTTCCTTTTGT-3′; for amplification of norB-aniA promoter region |
23 |
MFA8 |
5′-GGGATCCCGTCCCATTTTGAGAGCTCCTTTT-3′; for amplification of norB-aniA promoter region |
23 |
MFA10 |
5′-GTGCTGCAAGGCGATTAAGTT-3′; for checking orientation of insertion of norB-aniA promoter in lacZ fusions |
This work |