TABLE 1.
Strains, plasmids and primers used in this study
| Strain, plasmid, or primer | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli EL250 | F−mcr Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG [λcl857 araC-PBADflpe] | 30 |
| S. aureus | ||
| RN4220 | Restriction-deficient transformation recipient | Laboratory stock |
| SH1000 | Wild-type strain cured of prophages; rsbU+ | 20 |
| KC055 | katA::pAZ106 katA+ | This study |
| KC056 | perR::kan katA::pAZ106 katA+ | This study |
| KC067 | ahpC::tet katA::pAZ106 katA+ | This study |
| KC070 | ahpC::tet perR::kan katA::pAZ106 katA+ | This study |
| KC041 | ahpC::tet | This study |
| KS100 | katA::Tn917 (ery) | 53 |
| KC043 | ahpC::tet katA::Tn917 (ery) | This study |
| MHKA | sodA::Tn917 (ery) | 26 |
| KC075 | ahpC::tet sodA::Tn917 (ery) | This study |
| KC072 | ahpC::tet katA::Tn917 (ery) perR::kan | This study |
| MHK2 | fur::tet | Laboratory stock |
| KC044 | ahpC::tet fur::kan | This study |
| KC073 | katA::Tn917 (ery) fur::kan | This study |
| KC072 | ahpC::tet fur::kan katA::Tn917 (ery) | This study |
| GC3 | ahpC::tet katA::Tn917 (ery) pSK5630 | This study |
| GC2 | ahpC::tet katA::Tn917 (ery) pSK5630 (ahpC+) | This study |
| Plasmids | ||
| SABAC1 | S. aureus bacterial artificial chromosome containing genomic DNA from 457727 to 401407 (COL coordinates) | J. Iandolo, http://www.genome.ou.edu/staph.html |
| pGL400 | tet cassette-containing vector | J. Garcia-Lara, personal communication |
| pSK5630 | E. coli-S. aureus shuttle vector | 12 |
| pKC2 | AhpC + 1-AhpC + 2 ahpC gene and promoter region in pSK5630 | This study |
| Primers | ||
| AhpCtet1 | AACAAAGAAATCTTACCATTTACAGCGCAAGCTTTCGATCCACGGATTTTATGACCGATGAGAAG | This study |
| AhpCtet2 | AATCTAAACCAGGTTGCAATGTTTTAGCGCCTTCTTCCCAAGAAATCCCTTTGAGAATGTTT | This study |
| AhpC + 1 | AATGTTGGATCCTGTCAGTGAAATGAATG | This study |
| AhpC + 2 | TCACTCGGATCCTTCATCCATCTCGAAC | This study |
| Acn1 | TAAGCGTTTTCTAAGCATGATG | This study |
| Acn3 | ACGTGATGACATATAGTTTTTAG | This study |