TABLE 2.
Oligonucleotide primers for S. salivarius and S. vestibularis
| Locus | Accession no. | Primer function | Primer | Sequence (5′-3′) |
|---|---|---|---|---|
| ilvC | AF220670 | MLST | ilvC-up | GATCAGGTCACGATGTTAT |
| MLST | ilvC-dn | GGTGCATATCCAGCTTCAGT | ||
| ddlA | gbU69167 | MLST | ddlA-up | TCAAGTGTGGCTATGGA |
| MLST | ddlA-dn | GTAGATGGCTCCATCCTC | ||
| glcK | AF442552 | MLST | glcK-up | TGGGCAGAAACTCAAGA |
| MLST | glcK-dn | AACACCACCACCGATAAC | ||
| pyrE | CP000024 | MLST | pyrE-dn | CGCTTTACGGAGGAACAT |
| MLST | pyrE-up | GTCCGTCTGCAGTGATGT | ||
| thrS | CP000024 | MLST | thrS-up | ATCACTGAAGATGGAAGC |
| MLST | thrS-dn | CCAAGTTTACGGTGGTCA | ||
| dnaE | CP000024 | MLST | dnaE-up | GGACTGGGAGCCTGGGAT |
| MLST | dnaE-dn | ACTCCCTGCAGCAGACCC | ||
| pepO | AJ249396 | MLST | pepOup | AACTCTACCACCCTTATGA |
| MLST | pepOdo | GGTTTGTTCCACTTGCTCCAG | ||
| tkt | CP000024 | MLST (S. vestibularis) | tkt-up | GCAGCACCAATGGGTTAC |
| MLST (S. vestibularis) | tkt-dn | CCAAGTTTACGGTGGTCA | ||
| tkt-up2 | CTGGCCACCCTGGTGTGG | |||
| . | tkt-dn2 | GGAGCACCGTGAACACCG | ||
| tkt-up3 | AATGCTATTCGTTTTCTAGG | |||
| tkt-dn3 | TTGAAATCAGCATATACTT | |||
| tkt-up4 | CAGGTCATGGTTCAATGCT | |||
| tkt-dn4 | TGAATTACGAGTTGCTTG | |||
| tkt-dn5 | TTGATATCATTTGAATC | |||
| tkt-up6 | ATGGGTGCAGCACCAATGG | |||
| tkt-dn6 | ACCAATGACTGTCTTAAC | |||
| tkt region | Large region | tkt-dn7 | CTTAGAACCCCATTGACGG | |
| Large region | tkt-up8 | GGTGTACGTGAGTTTGCG | ||
| Large region | tkt-salup | CTTGGCATTGCTTGACTTCG | ||
| Large region | tkt-saldn | CCTTCGATTGAGATTAGTAAC | ||
| Large region | tkt-saldn8 | CAAAGGCAGCCTTGTATTCC | ||
| Large region | tkt-saldo9 | CAGAACCAGTCGCAATGA | ||
| Large region | tkt-vesup | CAAGATTATCTTGTCATCG | ||
| Large region | tkt-vesdo | GGCTGGAAAGTCCTTGCATAC | ||
| Large region | tkt-vesup9 | GGCAGCAAGTCTACCATGG | ||
| Large region | tkt-vesdo9 | GCTTGATACCATGCAGCC- | ||
| ilvC region | Large region | ilvB-up3 | GTGGACAGCTCAGTATTATC | |
| Large region | ilvC-dn3 | GCTTCTGCTACTGTGTAAG | ||
| Large region | ilvC-up5 | CTGAAGAAGATTTGTTTGGTG | ||
| Large region | tyrS-do4 | GGTAACATGACAGCAGGTAC | ||
| pepO region | Large region | pepO-up6 | GCAGGGTGCGTGCTCTTG | |
| Large region | pepO-up4 | ACGCTTACAAGATGATTT | ||
| Large region | pepO-dn6 | CTTCTGGAACGATGATTTTATC | ||
| Large region | pepO-dn4 | TCATAAGGGTGGTAGAGTT | ||
| Large region | pepO-dn5 | TACCAAATAATAACACGATC | ||
| Large region | pepO-dn7 | TAGTCATATGAAACTCCT | ||
| Large region | pepO-up5 | CTGGAGCAAGTGGAACAAACC | ||
| Large region | pepO-up7 | GAAATTTCAGTTGCTCATAG |