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RALP3 negatively regulates the GAS hyaluronic acid capsule and cysteine protease. A. Hyaluronic acid levels found in the supernatant and on the surface of bacteria measured by ELISA. B. Two-dimensional gel electrophoresis of proteins in the supernatant of WT M1 GAS and the ΔRALP3 mutant. C. Cysteine protease activity in supernatants from WT M1 GAS and the ΔRALP3 mutant as measured using a fluorescent casein substrate assay.
