FIG. 5.

PrgZ effects on p043lacZ induction ability of peptides. Synthetic cCF10 variant peptides were added at 5 ng/ml to cCF10-deficient strain JRC104 harboring p043lacZ with a second plasmid expressing PrgZ from pMSP6043-1 (Z⁺, dark bars) or a vector control, pMSP6043-2 (Z⁻, light bars). Peptides were added immediately after a 1:5 dilution from stationary-phase cultures, and β-galactosidase induction levels were measured after 90 min incubations. Data shown are representative of at least two independent experiments done in duplicate; error bars represent one standard deviation of the mean.