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Effects of MC treatment of exponentially growing cells on the excision frequency of ICESt1. Fragments containing the attB (361 bp) or attI (400 bp) recombination site were amplified using 1 μg template DNA from MC-treated cells and primers described in the legend to Fig. 1. An internal fragment of the fda gene (339 bp) was amplified as a control using 1 ng template DNA.
