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Effects of arp1 deletion on the excision frequency of ICESt1. Fragments containing the attB (361 bp) and attI (400 bp) recombination sites from the strains harboring wild-type ICESt1 (ICESt1 WT) or ICESt1 Δarp1 were amplified by PCR using primers described in the legend to Fig. 1 and amounts of template DNA ranging from 1 μg to 1 pg for CNRZ368 Δarp1 and from 1 μg to 1 ng for CNRZ368. An internal fragment of the fda gene (339 bp) was amplified as a control.
