FIG. 1.

Localization of the DivIVA protein in S. pneumoniae Rx1 cells. (A) Fluorescence micrographs showing the localization of DivIVA in representative cells at different stages of division, designated 1 to 6. Cells were stained to visualize DivIVA (green) and DNA (red); the merge images show the localization of DivIVA and DNA simultaneously. Scale bar = 0.25 μm. (B) Immunogold labeling of DivIVA. Exponentially growing cells were fixed and freeze-substituted. Thin sections were immunolabeled with specific antibodies and a gold-labeled secondary antibody and analyzed by electron microscopy. Dark grains show the localization of DivIVA. Scale bar = 0.25 μm.