TABLE 1.
Cloning primers used in this studya
| Primer | Sequence | Product size (bp) |
|---|---|---|
| spnF_divIVA | ATGCCAATTACATCATTAGAA | 848 |
| spnR_divIVA | AGTTTCTGTAGCTGGTGTTGGAC | |
| spnF_ftsA | ATGGCTAGAGAAGGCTTTTTTACAGG | 1,442 |
| spnR_ftsA | CTTGAGCAGCAGCTGTATCAAATG | |
| spnF_ftsZ | ATGACATTTTCATTTGATACAGCTG | 1,360 |
| spnR_ftsZ | CCACTCTCTCGATGAGCACTCA | |
| spnF_zapA | GTGAATTTTATGGCAAATCTAAAT | 337 |
| spnR_zapA | AAGACCAATAGAAGAAGGAATGA | |
| spnF_ezrA | TTGTTTCAAGTAAAAAAGGAGTTT | 1,787 |
| spnR_ezrA | TGCTCCTCACACAATAAAATCTT | |
| spnF_ftsK | ATGGCAAACAAGAATACAAGTAC | 2,330 |
| spnR_ftsK | TCCAAACTTGGAAAGAAGCTATT | |
| spnF_ftsL | ATGGCAGAAAAAATGGAAAAAAC | 347 |
| spnR_ftsL | ACTCTTTTTGTCCACTTCATATC | |
| spnF_ftsB | ATGTCTAAAAATATTGTAC | 369 |
| spnR_ftsB | TCACCTTTGAATCAAGTC | |
| spnF_ftsQ | ATGTCAAAAGATAAGAAAAATGAG | 1,234 |
| spnR_ftsQ | TGGTCACTAAATAACTATAAGAGA | |
| spnF_ftsW | ATGAAGATTAGTAAGAGGCACTT | 1,260 |
| spnR_ftsW | TGAAGAGACATAAACTATCCTTT | |
| spnF_pbp2X | ATGAAGTGGACAAAAAGAGTAAT | 2,280 |
| spnR_ pbp2X | ATTCCAGCACTGATGGAAATAAA | |
| spnF_pbp1A | ATGAACAAACCAACGATTCTGCG | 2,189 |
| spnR_ pbp1A | ATCACCCAGAAAAATCTGGATGA | |
| spnF_pcsB | ATGAAGAAAAAAATCTTAGCGTCA | 1,206 |
| spnR_ pcsB | AGGGCTCTATTCGAGTCCCT | |
| spnF_spo0J | ATGGAAAAATTTGAAATGATT | 760 |
| spnR_ spo0J | TTATTTCAGGCTGTTGATAAT | |
| spnF_pfrA | ATGGTCAACTATCCACATA | 597 |
| spnR_ pfrA | TCATCTTGTTTTACCACC | |
| spnF_lytB | ATGAATTTAGGAGAATTTTGG | 2,166 |
| spnR_ lytB | CTAATCTTTGCCACCTAGC |
a
Additional bases, containing the appropriate restriction sites, were added to the sequences to allow cloning in the different plasmid vectors, as described in the text. The product size is total length, including linkers.