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CovR activates dppA expression in vivo. RPA was performed on RNA isolated from the PdppA-gusA transcriptional fusion parent strain (JRS675, designated wt) and derivative strains ΔcovR (JRS679) and ΔcodY (JRS692). RNA was isolated from late-log-phase cultures and probed with gusA and dppA antisense radiolabeled RNA. A ribonucleotide size marker (M) (Ambion) is shown to the right of the image. nt, nucleotides.
