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Primer extension to map the 5′ end of the dppA transcript. Antisense primer PdppA PE3 was annealed to RNA isolated from JRS4 in late log phase, and primer extension was performed using Superscript II enzyme (Invitrogen). The arrow (+1) indicates the 5′ end of the transcript. Predicted promoter elements (−35 and −10) and the ribosome binding site (rbs) are labeled. CovR consensus binding sequences (CB) are shown in relation to the start of dppA transcription. ORF, open reading frame.
