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Identification of the dppA transcript by in vitro transcription. In vitro transcription was performed using plasmid pEU7526 (containing the PdppA region from positions −233 to +102) linearized separately with two different enzymes (EcoRI and XhoI) at sites downstream of the 5′ end of the dppA transcript (+1). A ribonucleotide size marker (M) (Ambion) is shown to the right of the image. ORF, open reading frame. nt, nucleotides.
