TABLE 1.

Primers used in this study

Primer	Sequence (5′-3′)a	Use
PdppA S1 BamHI	cggcgcggatccTGTATTAATGGCTATCTGAAG	PdppA-gusA transcriptional fusions and construction of pEU7535
PdppA A2 PstI	atagaaaactgcagTGATATTCCCTTGATTGTGG	Construction of pEU7535
PdppA A1 XhoI	gggccgctcgagCCCAGTCAAAAATAACGTGA	PdppA-gusA transcriptional fusions
PdppA PE3	TGATAATAGAGAAGTATTTTAGG	Primer extension
PdppA S mut CB-1	TGATCAGTTTCCTTCTTTTTTTAATCTAAAAT	PdppA CB-1 mutagenesis
PdppA A mut CB-1	AAAAAAAGAAGGAAACTGATCACTAAGAAATA	PdppA CB-1 mutagenesis
PdppA S mut CB-2	TTCTTTTTTTCCTCTAAAATAAGTCATGAAAC	PdppA CB-2 mutagenesis
PdppA A mut CB-2	TTATTTTAGAGGAAAAAAAGAATTAAACTGAT	PdppA CB-2 mutagenesis
PdppA S mut CB-3	CTTTTGTTCCTACATCAACTTATAAAAAAGCC	PdppA CB-3 mutagenesis
PdppA A mut CB-3	GTTGATGTAGGAACAAAAGATAAAGAGGTTTC	PdppA CB-3 mutagenesis
PdppA S mut CB-4	CAAAAATTCTCCTTAACCAGTTAAACAATTGC	PdppA CB-4 mutagenesis
PdppA A mut CB-4	AACTGGTTAAGGAGAATTTTTGTTATTTTCAG	PdppA CB-4 mutagenesis
PdppA S mut CB-5	TATTTATTAAGGAGAAATAAGGAGATTTGATTG	PdppA CB-5 mutagenesis
PdppA A mut CB-5	CCTTATTTCTCCTTAATAAATAATAATAGTAAC	PdppA CB-5 mutagenesis
codY S1 EcoRI	atccggaattcGTAAAATCACATCTATTTTGC	codY insertional inactivation
codY A1 SacI	gaatcgcgagctcATCTAACTCTCCAAGAATTGC	codY insertional inactivation
PdppA-M6-F	CCCTCCCAACAAGCTATTTTTGTTCGTAACC	RPA probe construction
PdppA-M6-R	GGGAGGGGTGACATCATTTCCTACAGGATAACC	RPA probe construction
Ptrc99a-EcoRI	gggaattccGTAGCGCCGATGGTAGTG	Construction of pEU7535
Ptrc99a-BamHI	gggggatccGCTTCCTCGCTCACTGAC	Construction of pEU7535

^aUppercase letters indicate sequences derived from the genes of interest, lowercase letters indicate sequences added for cloning purposes, and underlined sequences indicate restriction sites.