Time courses for cell growth, pH, and gelatinase activity of E. faecalis cultures with and without siamycin I (1 μM). E. faecalis OU510B was inoculated into fresh medium with (solid symbols) or without (open symbols) siamycin I (1 μM) and was then cultured. Culture supernatant was collected every hour, and the OD660 (triangles), pH (diamonds), and gelatinase activity at OD540 (circles) were determined. The data are averages ± standard deviations of duplicate determinations.