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Expected restriction fragment length polymorphisms of bfpA PCR amplicons
| bfpA allele | Digestion pattern (bp)a
|
||
|---|---|---|---|
| AluI | BfaI | BstNI | |
| α1 | 408 | 408 | 155, 253 |
| α2 | 408 | 75, 333 | 155, 253 |
| α3 | 408 | 75, 333 | 408 |
| β1 | 16, 54, 113, 231 | 39, 375 | 414 |
| β2 | 16, 170, 228 | 414 | 414 |
| β3 | 16, 54, 344 | 39, 375 | 414 |
| β4 | 16, 177, 215 | 39, 369 | 408 |
| β5 | 16, 54, 123, 215 | 39, 369 | 408 |
| β6 | 16, 392 | 39, 369 | 408 |
| β7 | 16, 54, 113, 225 | 39, 369 | 408 |
Underlined fragments are not detectable under standard electrophoretic conditions.
