TABLE 1.

Oligonucleotides used to confirm integration of the vectors pSM1-3 in the genes ccpA, hprK, and ptsH

Oligonucleotide	Sequence (5′→3′)	Target DNA
ccpA-1	TACAATGGTAGGTAGAGCC	Upstream region of ccpA
ccpA-2	TTGTTCTGAAATACGTTCGCC	ccpA
hprK-1	CTAAATAATATTTTGTAGCGAACAG	Upstream region of hprK
hprK-2	AAGTCTGCTTTCTAAGTAATTCG	hprK
ptsH-1	TCAATAATGTCTCCAACATGTGC	Upstream region of ptsH
ptsH-2	AGCAGATAAGCTTTCGCAATG	Downstream region of ptsH
pLSV101BamHI	AATAAGCTTGGCTGCAGGTC	Vector pLSV101
pLSV101EcoRI	GTTTTCCCAGTCACGACGTT	Vector pLSV101