TABLE 1.
Bacteria and plasmids used in this study
| Bacterium or plasmid | Relevant characteristics | Source |
|---|---|---|
| E. coli | ||
| 1830 | pro met Kmr Nmr; containing transposon Tn5 on the suicide plasmid pJB4JI | Gantotti et al. (7) |
| JM101 | supE thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZ ΔM15] | Messing et al. (15) |
| DH5 | supE44 hsdR17 recA1 endA1 gyrA1 thi-1 relA1 | Hanahan and Reusch et al. (8, 21) |
| E. carotovora subsp. carotovora | ||
| 89-H-4 | Putative biocontrol agent | Laboratory stock |
| H-rif-8-2 | 89-H-4; Rifr | This work |
| Ea1068 | Wild type | Laboratory stock |
| T-29 | Wild type | Laboratory stock |
| E108 | Wild type | Laboratory stock |
| A-100 | Wild type | Laboratory stock |
| 86-H-2 | Wild type | Laboratory stock |
| TH22-10 | H-rif-8-2; caroS1K:: Tn5 Rifr Kanr | This work |
| Plasmids | ||
| pACYC177 | Ampr Kanr; low copy number | Change et al. (2) |
| pBR322 | Ampr Kanr | Bolivar et al. (1) |
| pAYL4 | AmprcaroS1K caroS1I | This work |