TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| 269-492 | K. kingae clinical isolate | St. Louis Children's Hospital |
| ATCC 23330 | K. kingae nasal isolate | ATCC |
| ATCC 23332 | K. kingae blood isolate | ATCC |
| 97-982 | K. kingae clinical isolate | St. Louis Children's Hospital |
| 05-001-1818 | K. kingae clinical isolate | St. Louis Children's Hospital |
| KK01 | Nonspreading/noncorroding derivative of 269-492 | This study |
| KK03 | Spreading/corroding derivative of 269-492 | This study |
| 60H11T1 | Noncytotoxic transposon mutant of 269-492 | This study |
| 67A6T1 | Noncytotoxic transposon mutant of 269-492 | This study |
| 97G1T1 | Noncytotoxic transposon mutant of 269-492 | This study |
| ATCC 33394 | K. denitrificans type strain | ATCC |
| ATCC 51147 | K. oralis type strain | ATCC |
| DH5α | E. coli F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE441 thi-1 gyrA96 relA1 | 17 |
| RTXAC | DH5α containing rtxAC in pRTXAC | This study |
| WAM716 | DH5α containing rtxBD in pWAM716 | 5 |
| RTXAC/WAM716 | DH5α containing pRTXAC and pWAM716 | This study |
| Plasmids | ||
| pWAM716 | rtxB and rtxD from E. coli in pACYC184 | 5 |
| pRTXAC | rtxA and rtxC from K. kingae cloned into pTrc99A | This study |