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. 2006 Nov 10;189(2):551–560. doi: 10.1128/JB.00986-06

FIG. 1.

FIG. 1.

Confirmation of rpoE disruption in the mutant strain. (A) Southern blot analysis of X. fastidiosa J1a12 and rpoE strains. Total DNA was digested with EcoRI and hybridized with a probe comprising the coding region of rpoE. The original 5.0-kb fragment in J1a12 was divided into two fragments of 1.3 kb and 6.8 kb by insertion of the vector DNA. (B) A polyclonal antiserum raised against X. fastidiosa RpoE was used in an immunoblot assay of the J1a12 and rpoE strains and the mutant strain complemented with a copy of the rpoE gene in trans [rpoE(pRPOE)]. The arrowhead indicates the band corresponding to the RpoE protein.