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. 1999 Sep 14;96(19):10863–10868. doi: 10.1073/pnas.96.19.10863

Figure 6.

Figure 6

Effect of pretreatment with inhibitory anti-gelatinase B antibodies on the percentage of circulating CD34+ mononuclear cells at steady state (T = 0) and at 15 min after (T = 15) a single injection of IL-8 (0.1 mg/kg) (animal D). After erythrocyte lysis, peripheral blood leukocytes were stained with FITC-labeled anti-CD34 and analyzed by flow cytometry. The number of CD34+ cells is shown as percentage of the total number of appropriately gated cells. IL-8 induced an increase in circulating CD34+ cells from <0.1% to 0.5% at 15 min. After pretreatment with anti-gelatinase B antibodies (2 mg/kg), no increase of CD34+ cells was observed.