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Repression of bio operon transcription upon overproduction of AccC. The plates are MacConkey agar plates containing 100 μM IPTG, and the biotin concentrations are indicated below the plates. The red color is due to depression of the bioBFCD promoter that drives expression of the lacZ and lacY genes (4). The host was strain CY1740, and the accC and vector plasmids were pCY701 and pKK223-3, respectively. Essentially identical results were obtained with strains BM2661 and CY486.
