FIG. 1.
Antisera generated in this study to probe for putative OMPs and IMPs. Proteins were separated by SDS-PAGE and transferred to nitrocellulose. m, prestained molecular mass standards with sizes (in kDa) noted on the left side of each immunoblot; L, whole-cell lysate of F. tularensis LVS; S, whole-cell lysate of F. tularensis Schu S4; rP, purified recombinant His6 fusion protein. Immunoblot assays were performed by probing with antisera against putative OMPs (Pal, TolC-A, PilQ, SilC, FtaG, TolC-B, FopA, Mip, YapH-N, YapH-C, YapH-LVS, Cht1, SrfA, Tul4-A, Tul4-B, and LamB) and IMPs (SecY and LolC).