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FtsN overexpression restores localization of FtsL and FtsQ in cells in which FtsK is depleted at 37°C but not at 42°C. FtsK was depleted in isogenic FtsN-overexpressing cells (↑FtsN) and control cells (wt) at 37°C or 42°C for 3 to 5 h until control samples showed filamentation. Localization of GFP fusions to FtsQ and FtsL (expressed from λatt) is shown along with corresponding phase-contrast images. Expression of FtsN was from pDSW204.
