TABLE 1.
Constructa | Mutant background | Temp (°C) | Growth on:
|
||
---|---|---|---|---|---|
Ara | Glc | Glc + IPTG | |||
GFP-FtsN | pBAD-ftsKb | 37 | +++e | ++ | +++ |
GFP-FtsN | ΔftsKc | 37 | − | − | + |
GFP-FtsL | pBAD-ftsK | 37 | +++ | − | − |
GFP-FtsN | pBAD-ftsK | 42 | +++ | − | − |
GFP-FtsN | pBAD-ftsNd | 42 | +++ | +++ | +++ |
All GFP constructs were expressed from a single-copy pDSW204 promoter.
The FtsK depletion strain was ΔftsK::cat-Δ5 complemented by pJC85 (pBAD42-ftsK).
ΔftsK::cat-Δ5 with no complementing plasmid.
FtsN depletion strain.
+++, wild-type growth; ++, slightly wrinkled colonies; +, poor growth and wrinkled colonies; −, no growth.