TABLE 2.

FtsN swap suppression

Constructa	Mutant backgroundb	Temp (°C)	Complementation with IPTG at a concn of c:				Notesd
			1 μM	10 μM	100 μM	1,000 μM
FtsN	pBAD-ftsN	37	+++	+++	+++	+/−
FFN	pBAD-ftsN	37	+++	+++	+++	NDe
FtsN241	pBAD-ftsN	37	+++	+++	+++	ND
FtsN	ΔftsK	37	+	+	−	−	P1
FFN	ΔftsK	37	−	−	−	−	P1
FtsN241	ΔftsK	37	+	+	−	−	P1
FtsN	pBAD-ftsK	37	++	++	−	−
FFN	pBAD-ftsK	37	−	−	−	−
FtsN241	pBAD-ftsK	37	++	++	−	−
FtsN	ftsQ1(Ts)	42	+	+/−	−	−
FFN	ftsQ1(Ts)	42	−	−	−	−
FtsN241	ftsQ1(Ts)	42	+/−	−	−	−

^aConstructs were expressed with a C-terminal 3×Myc epitope from the multicopy pDSW204 plasmid.

^bSame mutant backgrounds as those in Table 1.

^c+++, wild-type growth; ++, slightly wrinkled colonies; + and +/−, very poor growth and small wrinkled colonies (+ indicates that there was efficient recovery of transductants); −, no growth.

^dP1, tested by P1 transduction.

^eND, not done.