TABLE 1.
Primers used in this study
| Primera | Sequence (5′→3′)b | Description |
|---|---|---|
| F1 | GAATACAATTCAACCTGCT | Amplifies 435-bp meningococcal rp1O ORF |
| R1 | ATTTCAATCTTACCACCAA | |
| F2 | GTTAAAAGCCTGATTGGTA | Amplifies 186-bp meningococcal rpmD ORF |
| R2 | AAGACTCCACTTTCAACAA | |
| F3 | GTTAACTCCCAAAATGTCTT | Amplifies 519-bp meningococcal rpsE ORF |
| R3 | CAAAACATGAAATTGAAGAA | |
| F4 | CGGGATCCCGTACAGCTCGCTTCTGAAAT | Amplifies 613-bp fragment of the meningococcal secY ORF |
| R4 | CCAAGCTTGGCTATTTGTATCAGCCGAACC | |
| F5 | GAAGATTTTGGATTTGTTCG | Amplifies 339-bp fragment of the meningococcal fur ORF |
| R5 | CACACGCCGTACATATAAAG | |
| F6 | TCAAGCTCTTTAGGTTCTGC | Amplifies 347-bp fragment of the meningococcal nspA ORF |
| R6 | ATGTAGTTGTAGCGGTAGCC | |
| F7 | ATACGGTTGAAGTGGAAT | Amplifies 520-bp fragment of the meningococcal aniA ORF |
| R7 | AACATAAACTTTGTCGAAGA | |
| F8 | CGGGATCCCGCAAGGTTTCATTTAATAAG | Amplifies 111-bp promoter fragment of the meningococcal secY/ribosomal operon |
| R8 | CGGGATCCCGATCTAAGATGGTCTGCATTT | |
| F9 | GCTCTAGAGCAAAATATTGCGATGCAAAA | Amplifies 101-bp promoter fragment of the meningococcal nspA gene |
| R9 | CGGGATCCCGATATTTTGGTTCCTTTATGG | |
| F10 | CGGGATCCCGCTGCTTCTTTATAGTGGAGA | Amplifies 114-bp promoter fragment of the meningococcal rmp gene |
| R10 | CGGGATCCCGCCTCATTAAATTTGTACAGC |
a
F, forward; R, reverse.
b
Restriction sites are underlined.