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Time course of phosphorylation of CbbRR1 (▪) and H171DCbbRR1 (□) catalyzed by D696NCbbSRR96 (▵) (A and B) and D696NCbbSRT189 (○) (C and D). Reaction conditions were as described in the legend of Fig. 4. Quantitation of phosphorylated products from time courses (left) was obtained from phosphotransfer reactions depicted in gels (right). The arrow indicates where CbbRR1 preparations were added to the reaction mixture.
