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Western Blot analysis of toxins from VGS strains TIGR4 (T), R75II (75), R76 (76), and the negative control NCTC 10712 (NC). The samples were cell lysates from 15 ml of mid-log-phase cultures. Pneumolysin was detected with an anti-rabbit pneumolysin PAb (Ply) and an anti-mouse pneumolysin MAb (PLY-7).
