TABLE 1.
Synthetic oligonucleotide primers used for sequencing of the ply gene
| Primera | Sequence (5′→3′) |
|---|---|
| 27S | TACTTAGTCCAACCACGG |
| 27R | CTTGGCTACGATATTGGC |
| 4W | GATCATCAAGGTAAGGAA |
| 4V | CAATACAGAAGTGAAGGCGG |
| 4T | GTTGATCGTGCTCCGATGAC |
| 9Y | CGGGATCCGGCAAATAAAGCAGTAAATGACTTT |
| 27T | ATAAGTCATCGGAGCACG |
| 15C | GGAGGTAGAAGATGGCAAATAAAGC |
| 15D | CTAGTCATTTTCTACCTTATCCTCTACC |
a
Primers 27S and 27R were used for sequencing of the toxin gene in strains R75I, R76, R77, R84, COL15, and TIGR4. Primers 15C and 15D were used for sequencing of the toxin gene in strain R75II.