FIG. 8.

UO126 specifically inhibited viral genomic RNA replication. (A) Schematic diagram of the DI RNA-CAT reporter construct. Two primers used for RT-PCR are indicated with arrows, and the size of the expected RT-PCR product is also shown. (B) BHK cells were cotransfected with MHV-JHM viral genomic RNA and in vitro-transcribed 25CAT DI RNA. Cells were treated with UO126 (50 μM) or DMSO at 5 h posttransfection and then harvested at 12 and 24 h posttransfection. Intracellular RNAs were extracted and RT-PCR was performed to measure the genomic DI-RNA replication. β-Actin was used as an internal control. (C) CAT activity. The experiments were done as in panel B, except that the cell lysates were used for the CAT assay. Values are means of three independent experiments. Error bars represent the standard deviations.