FIG. 5.
Electron microscopy of L47A mutant-infected cells. HFF cells infected with wild-type (A) or with the L47A mutant (B to D) bacmidgr viruses were fixed, stained, and embedded and thin-sectioned in situ as described in Materials and Methods. “Double-ringed” (large arrows) and empty (small arrows) nuclear capsids typical of infections with wild-type virus are seen in panel A. More irregular-looking capsids (e.g., top-left corner, Panel B) and “tubules” (arrows) are seen in panels B to D. The inset (bottom left corner of panel C) shows tubules sectioned perpendicular to the horizontal plane of cell growth seen in the other panels. (D) Immunoelectron micrograph prepared after reaction with rabbit antibodies to pAP and then 10-nm-gold (black dots)-tagged secondary antibodies.