FIG. 2.

Detection of FIV Gag- and Env-specific CTL following inoculation. Virus-specific CTL responses were measured in restimulated lymphocytes isolated from peripheral blood at 2 weeks (A) and 6 weeks (B) following immunization. Autologous skin fibroblasts served as target cells and were infected with vFIV-env or vFIV-gag or with wild-type (data not shown) vaccinia virus stocks. Release of lactate dehydrogenase was measured to assay specific lysis as described in Materials and Methods. Results shown are the mean values for triplicate cultures at an effector-to-target cell ratio of 12.5:1 or 25:1 and represent specific lysis values measured for FIV-Env or FIV-Gag target cells after values measured for targets infected with wild-type vaccinia virus are subtracted. A significant CTL response was defined as a value greater than or equal to 10% lysis. An asterisk indicates that data for that cat are not available.