FIG. 4.

CUL5 NTD inhibits viral late gene expression. (A) Duplicate independent cultures of HeLa cells were preinfected with the indicated Ψ5 vectors (multiplicity of infection = 50) and superinfected with Ad5. Extracts prepared 24 h later were Western blotted with anti-Ad5 serum. Major virion proteins are shown. (B) Extracts prepared from HeLa cells 24 hpi with Ad5 or dl1520 were analyzed as in panel A. (C) Four cultures of HeLa cells were preinfected with the empty Ψ5 vector or Ψ5-NTD for 16 h and superinfected with either Ad5 or dl1520 for 24 h as indicated at the top. Cell extracts were prepared and analyzed as in panel A.