TABLE 2.
Primer use and name | Sequence 5′ to 3′ | Amplicon size (bp) |
---|---|---|
Quantitative RT-PCR | ||
L5 Forward | CGGAGACAAAACTAAACCTGTAACAC | 102 |
L5 Reverse | TCCCATGAAAATGACATAGAGTATGC | |
L3 Forward | CGCTTTCCAGGCTTTGTTTC | 63 |
L3 Reverse | GCGACCGGCCGTATTG | |
L2 Forward | CGGTCTTTGTGGTTCTTGCA | 79 |
L2 Reverse | TGCATTCTTCCTCGGAATCC | |
5′ EF2 promoter | CCTTCGCAGCGCAGTCACA | 146 |
3′ EF2 promoter | GGCGGTGGATTCTCCCAGGT | |
Kanamycin forward | CGAGTGATTTTGATGACGAGCGT | 93 |
Kanamycin reverse | CGACTGAATCCGGTGAGAATGG | |
Site-directed mutagenesis | ||
FwdVtoRLtoD | CTGACTCTGAGAAATACAGAGAGCAGGATCTTACACTATTTAATAG | |
RevVtoRandLtoD | CTATTAAATAGTGTAAGATCCTGCTCTCTGTATTTCTCAGAGTCAG | |
Fwd Stop Cul5 | GCTACCATATTTAAATAATAATTACCTTTGAAGCAGAAGGGGGTGGG | |
Rev Stop Cul5 | CCCACCCCCTTCTGCTTCAAAGGTAATTATTATTTAAATATGGTAGC |