TABLE 2.
Sequences of primers used in this study
| Primer use and name | Sequence 5′ to 3′ | Amplicon size (bp) |
|---|---|---|
| Quantitative RT-PCR | ||
| L5 Forward | CGGAGACAAAACTAAACCTGTAACAC | 102 |
| L5 Reverse | TCCCATGAAAATGACATAGAGTATGC | |
| L3 Forward | CGCTTTCCAGGCTTTGTTTC | 63 |
| L3 Reverse | GCGACCGGCCGTATTG | |
| L2 Forward | CGGTCTTTGTGGTTCTTGCA | 79 |
| L2 Reverse | TGCATTCTTCCTCGGAATCC | |
| 5′ EF2 promoter | CCTTCGCAGCGCAGTCACA | 146 |
| 3′ EF2 promoter | GGCGGTGGATTCTCCCAGGT | |
| Kanamycin forward | CGAGTGATTTTGATGACGAGCGT | 93 |
| Kanamycin reverse | CGACTGAATCCGGTGAGAATGG | |
| Site-directed mutagenesis | ||
| FwdVtoRLtoD | CTGACTCTGAGAAATACAGAGAGCAGGATCTTACACTATTTAATAG | |
| RevVtoRandLtoD | CTATTAAATAGTGTAAGATCCTGCTCTCTGTATTTCTCAGAGTCAG | |
| Fwd Stop Cul5 | GCTACCATATTTAAATAATAATTACCTTTGAAGCAGAAGGGGGTGGG | |
| Rev Stop Cul5 | CCCACCCCCTTCTGCTTCAAAGGTAATTATTATTTAAATATGGTAGC |