FIG. 2.

HIV infection of splenocytes and thymocytes of WT and huCD4/CXCR4 Tg mice. Splenocytes or thymocytes from WT mice, Tg mice, or human PBMCs were isolated and infected with equivalent amounts of DNase-treated NL4-3 virus at 37°C for 6 h. After 6 h, the viruses were removed and the cells were treated with trypsin (500 μg/ml) at 37°C for 10 min and then washed in growth medium. (A and B) Splenocytes were harvested at 24 h postinfection. Total DNA was extracted from the cells and subjected to PCR analysis with a primer pair for R/U5 (A) or R/gag (B). The reaction was carried out using the DNA preparation from 1 × 10⁵ cells (×1) or 1 × 10⁴ cells (×10⁻¹). In panel B, the lanes marked 5 × 10² and 5 × 10³ represent PCR products using 5 × 10² or 5 × 10³ copies of the pNL4-3 plasmid as the template.