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Effect of activation on HCV-specific CD8+ T cells. PBMCs were stimulated with epitope-specific peptide and cultured for 7 days. Analyses of CD127 and CD38 expression on HCV-specific CD8+ T cells are shown for two patients. In both, a CD127+ CD38− phenotype prior to stimulation was switched to a CD127− CD38+ phenotype after stimulation. Gates were set on the HCV-specific CD8+ tetramer-positive population.
