FIG. 2.

(A) Effect of UV inactivation of EBV preparation on expression of BZLF1 and EBNA2 mRNAs after EBV infection. EBV-negative Daudi and Akata cells and primary B lymphocytes were infected with UV-treated EBV. At the designated time of incubation, expression of BZLF1 and EBNA2 mRNAs was examined by RT-PCR. (B) Effect of anisomycin, an inhibitor of protein synthesis, on expression of BZLF1 and EBNA2 mRNAs after EBV infection. EBV-negative Daudi cells were preincubated for 3 h in a medium containing 25 μg/ml anisomycin. The culture was then centrifuged, and the cell pellet was resuspended in virus solution containing anisomycin and incubated for 90 min. Thereafter, the cells were resuspended in fresh medium containing anisomycin, incubated for the designated time, and examined for expression of BZLF1 and EBNA2 mRNAs by RT-PCR. The effect of anisomycin on expression of the β-actin protein was examined by immunoblot analysis. (C) Effect of actinomycin D, an inhibitor of RNA synthesis, on expression of BZLF1 mRNA after EBV infection. EBV-negative Daudi cells were infected with EBV in the presence of 5 μg/ml actinomycin D, incubated for the designated time, and examined for expression of BZLF1 mRNA by RT-PCR.