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In vitro growth characteristics of E2 individual (A) and multiple (B) glycosylation mutants and parental BICv. Primary swine macrophage cultures were infected (MOI of 0.01) with each of the mutants or BICv and virus yield titers determined at various times postinfection in SK6 cells. Data represent means and standard deviations from two independent experiments. Sensitivity of virus detection was ≥log10 1.8 TCID50/ml.
