FIG. 3.

Plaque formation of E2 glycosylation mutants and BICv. SK6 monolayers were infected, overlaid with 0.5% agarose, and incubated at 37°C for 3 days. Plates were fixed with 50% (vol/vol) ethanol-acetone and stained by immunohistochemistry with MAb WH303 (34).