FIG. 11.
L particles contain a 58-kDa, but not the 59.5-kDa, form of Vhs. [35S]methionine-labeled virions (lanes 1 and 2) and L particles (lanes 3 and 4) were purified from the extracellular medium from cultures of BHK-21 cells infected with wild-type HSV-1. Proteins were resolved by SDS-PAGE and transferred to an Immobilon P membrane, after which the membrane was subjected to autoradiography (A) (lanes 1 and 3) to detect all labeled viral proteins or probed using a UL41-specific antiserum (W) (lanes 2 and 4) for immunologic detection of the Vhs polypeptides.