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. 2006 Nov 8;81(3):1148–1161. doi: 10.1128/JVI.01812-06

FIG. 5.

FIG. 5.

The 59.5-kDa form of Vhs is enriched in the nucleus. Vero cells were harvested 8 h after infection with 20 PFU/cell of wild-type HSV-1 and separated into nuclear, nuclear wash, and cytoplasmic fractions. Aliquots of fractions from equal numbers of cells were analyzed by SDS-PAGE and Western blotting to detect the 59.5-kDa and 58-kDa forms of Vhs. The relative amounts of the 59.5-kDa and 58-kDa polypeptides were quantified for each fraction by densitometric scanning of the film resulting from the Western blot, and the results are shown in the four lines beneath the gel. Line 1 shows the percentage of the total amount (59.5 kDa plus 58 kDa) of Vhs present in each fraction. Line 2 shows the ratio of the amounts of the 59.5-kDa and 58-kDa polypeptides in each fraction. Line 3 shows the percentage of the total amount of the 59.5-kDa polypeptide that was present in each fraction, and line 4 shows the percentage of the total amount of the 58-kDa polypeptide in each fraction.