FIG. 4.

The TLR3 pathway mediates the phosphorylation of NF-κB/RelA at serine 276. (A) A549 cells were transfected with control siRNA (Con) or TLR3 siRNA, and a luciferase reporter plasmid containing the PRDII domain was cotransfected for 48 h. Cells were infected with RSV for 0, 9, or 18 h before cell lysis was performed. Shown is normalized luciferase activity expressed as change (n-fold) relative to that of uninfected cells. *, P < 0.05 (Student's t test). Error bars indicate standard deviations. (B) A549 cells were transfected with control siRNA (Con) or TLR3 siRNA (TLR3) and then RSV infected for 0, 9, or 18 h. Western immunobloting was performed to detect changes in phospho-Ser276 RelA (top panel), phospho-Ser536 RelA (middle panel), and RelA (bottom panel) levels using 100 μg of whole-cell extract.