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. 2006 Nov 15;81(3):1534–1536. doi: 10.1128/JVI.02099-06

FIG. 1.

FIG. 1.

Inhibition of proteasome activity preferentially enhances cellular susceptibility to infection by Nef-defective HIV-1. (A and C) HeLa-P4 cell cultures, preincubated for 1 h with the indicated inhibitor, MG132 (25 μM), lactacystin (25 μM), or E-64 (40 μM), were inoculated with dilutions of wild-type (R7 and R8) HIV-1 or the Nef-defective mutants (R7Nef− and R8Nef−). Viruses and drugs were removed 2 h postinoculation, and the cultures were replenished with fresh medium. Cultures were fixed and stained with X-Gal 2 days later, and the infected cells were quantified. Infectivity is expressed as the number of X-Gal-positive cells per nanogram of p24 in the inoculum. Panels B and D show the ratios of wild-type to Nef-defective HIV-1 infectivity determined in the presence (+) and absence (−) of the inhibitors. Error bars indicate standard deviations.