FIG. 2.

Relative rqRT-PCR analysis of MdBV PTP family members ptp-H1, -H2, -H3, -H5; -J1 to -J4; and -N1 to -N3 in gut (GT), fat body (FB), hemocytes (HE), nervous system (NS), and salivary glands (SG) from parasitized P. includens. All PTPs were expressed in one or more types of infected host tissue. Differences in total RNA amounts that were reverse transcribed and added to each rqRT-PCR mixture were normalized relative to an endogenous 18S rRNA control. The relative transcript abundance of each PTP is then reported in comparison to the gut sample, which is standardized to a value of 1.0 (see Materials and Methods). PCR products were run on 1% agarose gels to verify the size of each amplicon and to assure that no cross-amplification occurred. All treatments were replicated four times with tissues collected from different virus-injected larvae. Error bars for each sample equal 1 standard deviation.