TABLE 1.
Gene-specific primers used for real-time PCRs
| Family member | Forward and reverse primer sequences (5′-3′)a | Expected product size (bp) |
|---|---|---|
| ptp-H1 | (F) TATATAATGCGTTATCACAAGTGCG | 201 |
| (R) ACCTTGTCTTGGAATGCAGAGAAT | ||
| ptp-H2 | (F) CGTTGCAGCAGACAGCCTAC | 187 |
| (R) CTTAAGTGAGTTTCTATTTGGGTGGT | ||
| ptp-H3 | (F) CAGGCTATTGTTTCGAAATCTTTAAC | 194 |
| (R) GGAGTTATCCCAACACGTTACATCA | ||
| ptp-H5 | (F) GATCCATCACATATAACCCTAACACC | 258 |
| (R) TCTTCCAAATATCTCCATACCTTCC | ||
| ptp-J1 | (F) CCAATTCGGAAGGGTCTCG | 198 |
| (R) GGGGTAGCACTTTTGTTTGTTATCT | ||
| ptp-J2 | (F) TATACGCTGATTTAAGAAAGCATAAGAC | 212 |
| (R) GACATAATCATTTAATCGGTTCATACAA | ||
| ptp-J3 | (F) TGGAACATCCGGTGTCATTATC | 236 |
| (R) TTTGGTGTAAATGTACTTCCGCTT | ||
| ptp-J4 | (F) ATAAGCTATCTGCACGAAACTCCC | 201 |
| (R) CATAATTCTCCGGCTGACTAATGG | ||
| ptp-N1 | (F) TGTGGTACATTGCAGTGACGG | 174 |
| (R) GTTGTAATATTAAGTAATCATCTGGCG | ||
| ptp-N2 | (F) AGAAGAGTTTCATTATATCGTGCTCC | 143 |
| (R) CGCCGTTTTTCGACTCTATCA | ||
| ptp-N3 | (F) CTACAACATATCTGCATCATCATCAA | 203 |
| (R) CCTCAAAGCTAAAGAAATCTAATTGC |
a
F, forward; R, reverse.