FIG. 2.

RV39 internalization and colocalization with phospho-Tyr416 Src (upper panel) and phospho-Ser⁴⁷³Akt (lower panel) are blocked by chemical Src inhibitors. 16HBE14o− cells were incubated with sham or RV39 at an MOI of 100. RV39 (shown in green) induced Tyr⁴¹⁶ phosphorylation of Src (red, upper panel) and colocalized with Src (yellow). The high-affinity Src inhibitor PP2 blocked pTyr⁴¹⁶ Src colocalization of RV but did not block virus binding. The low-affinity inhibitor PP3 did not block the association of RV and Src. RV39 also induced Ser⁴⁷³ phosphorylation of Akt (red, lower panel) and colocalized with an Akt fraction (yellow). PP2 appeared to block Ser⁴⁷³ Akt phosphorylation and the colocalization of RV and Akt. The low-affinity inhibitor PP3 did not block the association of RV and Akt. LY294002 also appeared to block virus internalization and colocalization with phospho-Ser⁴⁷³Akt.