FIG. 2.

Accumulation of positive (A and B)- and negative (C)-strand RNA of HcRNAV in H. circularisquama cells inoculated with HcRNAV34 and HcRNAV109 virions. Total RNA was extracted from H. circularisquama cells immediately after inoculation (0 h) and at 24, 48, and 72 hpi; separated by gel electrophoresis; and blotted onto membranes. The membranes were then probed with strand-specific, DIG-labeled RNA probes. The same amount of genomic RNA of HcRNAV34 or HcRNAV109 was used as a positive control. RNA length is indicated on the right. Total RNA stained with SYBR gold is shown in panel D.